Tools such as the Functional Movement Screen (FMS) and Y-Balance Test Lower Quarter (YBT-LQ) have been used to assess quality of functional movement. To date, research has been inconclusive regarding the association between performance on the FMS and YBT-LQ and lower extremity injury incidence in collegiate soccer athletes. The purpose of this retrospective study was to explore whether functional movement, as measured by the FMS and Y-balance tests, in junction with athlete demographics, is associated with lower extremity injury. The study included retrospective data collected on 143 men’s and women’s soccer athletes over three years (2014-2016) from NCAA Division III Lebanon Valley College located in south-central Pennsylvania. Using chi-square tests and Mann Whitney U tests, functional movement and demographic data were compared to determine if differences existed in the data in relation to injury categories. In addition, gender differences between the FMS and YBT-LQ were explored. Gender, body mass index, injury history, FMS composite score, and YBT-LQ reach asymmetries were not associated with lower extremity injury. Additionally, the scores were not related to lower extremity noncontact injury categories. There were no significant differences between genders in YBT-LQ reach asymmetries or in the FMS CS. However, significant differences between genders were noted on ASLR and trunk stability push-up components of the FMS. Future research should seek to establish population-specific normative data for, and clinical utility of, the FMS and YBT-LQ. Clinicians should use caution in using any of these factors in isolation in clinical decision making with regard to injury prevention and return to play after injury.