The proposed research explores the structural differences of the chiral centers between two enantiomers and how they can be used to accomplish a successful separation. The methods for separating chiral mixtures represent a great importance for drug design or special material synthesis. The non-essential amino acid L-carnitine carries out a remarkable function in the human body, the most understood function being the transport of fatty acids in the mitochondria for oxidation1. L-Carnitine has one chiral center and can only be used by mammals in the L form. The method used for the separation of a racemic mixture of D/L carnitine was directly modifying the silica gel (SIG). The modified silica gel can be applied on a thin layer chromatography (TLC) plate or as a packing material for a high-performance liquid chromatography (HPLC) column. A TLC plate is a sheet of glass coated with a thin layer of adsorbent material. It is normally used for separation of molecules of different polarities. Polar molecules travel upwards, while nonpolar molecules stay at the bottom of the plate.2 We’ve explored the modifications of SIG to separate an achiral mixture of amino acids on a functionalized silica gel TLC plate as our starting point and have achieved success as a part of the learning of the process necessary to achieve such modification. We were able to make blank glass TLC plate and coat them with functionalized silica gel. We spotted a set of unfunctionalized silica gel TLC plates and a set of functionalized silica gel TLC plates with the same amino acids. During the application of slurry, the layer turned out to be too thick, therefore we could see the solute traveling upwards on the unfunctionalized silica gel TLC plate, but not on the functionalized one.