Collagen, the protein that makes up the majority of extracellular matrix structure, is essential in cell communication and proliferation. Research has found that collagen is also responsible for promoting many types of cancer growth because it both provides the infrastructure needed for the cells to expand, and it lessens the need for cell-cell interactions, therefore promoting cell motility. Clinically, collagen is used to treat severe burn victims as it assists in regenerating skin—this therapy typically involves bovine collagen or synthetic collagen. While these forms of collagen are readily available to clinicians, they are expensive, and it would be extremely useful to identify lower-cost alternatives. Recently, it has been discovered that tilapia, a fish popular for dietary consumption, and is now farmed all over the world, possesses high concentrations of collagen in its skin that can be easily extracted. Our hypothesis is that tilapia collagen could serve as a substitute to collagen derived from other sources in cell culture. By examining how malignant cells respond to tilapia collagen, we can determine if adding collagen to the media and/or as a coating on the surface of the tissue culture flasks in which these cells are grown on, creates a more “authentic” environment for the cells to grow in, better mimicking what cells would encounter in vivo. Firstly, we employ a cell-proliferation assay, comparing the impact that tilapia-derived collagen has on cell proliferation rate, with the findings providing insight into both cell growth and wound healing. We also utilized colony-forming assays to determine whether cancer cells respond differently to cancer drugs in the presence of collagen. This would give insight into the feasibility of using tilapia collagen in further cancer research without significant changes to traditional cell culture.