As rates of overweight and obesity increase in the United States, health care providers will interact with more patients with these conditions. Health care providers frequently make negative attributions about patients with overweight and obesity, and they may blame patients for their condition. This has negative implications for patients, including health care avoidance and weight gain. Limited research examines negative attitudes and beliefs among pre-health professional students, and even less research examines how stigmatizing attitudes vary based on patient BMI. This study hypothesized that pre-health care professional students’ weight stigmatizing attitudes and beliefs, perceptions of blame and responsibility, and motivation to treat would be greater as patient weight increased. Pre-health care professional students (n = 127) read a vignette depicting a patient who was overweight, obese, or severely obese and then completed questionnaires. Results suggested that pre-health professional students in the severely obese condition reported significantly higher negative weight-based attitudes and beliefs compared to participants in the overweight and obese conditions. No differences were observed in participants’ attributions of blame, responsibility, or in motivation to treat. Health care professionals may start to develop stigmatizing attitudes and beliefs about weight during education, so intervention should occur before individuals transition to practice.