Flowers of Saponaria officinalis exhibit protandry and floral color change: in early stages of anthesis, flowers bloom white and are male, then the petals turn pink as they transition to female. The depth of pinkness is determined by genetic and environmental factors. The pink color is due to the accumulation of anthocyanidin pigments, which are created through a biochemical pathway, each step catalyzed by enzymes, whose production is controlled by genes. Variation among plants in expression of these genes may lead to differences in final color or the rate that color change occurs. If these morphological differences result in fitness variation among individuals, the differences in gene expression may be subject to natural selection. Therefore, the pattern and extent of gene expression may represent an adaptive trait. This experiment quantified Dihydroflavonol 4-reductase gene (DFR) expression using quantitative polymerase chain reaction. First, gene expression was measured in samples obtained from previously collected late-stage female flowers, all which had their color quantified. Next, DFR expression was measured in flowers collected at five different life stages from ten different plants. We found DFR expression has no significant correlation with the level of pinkness reached in the female stage flower but does significantly increase as plants change from male to female.